The role of sphingomyelin breakdown in measles virus immunmodulation.

Role of Interferon in Replication of Virulent and Attenuated Strains of Measles Virus

Construction of a Minigenome Rescue System for Measles Virus, AIK-c Strain

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

Excessive Attenuation of Measles Virus as a Possible Cause of Failure in Measles Immunization

Ebolavirus requires acid sphingomyelinase activity and plasma membrane sphingomyelin for infection.

Acid sphingomyelinase (ASMase) converts the lipid sphingomyelin (SM) to phosphocholine and ceramide and has optimum activity at acidic pH. Normally, ASMase is located in lysosomes and endosomes, but membrane damage or the interaction with some bacterial and viral pathogens can trigger its recruitment to the plasma membrane. Rhinovirus and measles viruses each require ASMase activity during earl...

APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES

Measles is one of the most contagious human diseases. Although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. Therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. Serum neut...